FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158222
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC14700.035.766411
TCGCTCT9950.027.4972634
GTCTTAC8500.027.1144541
GTCTCGC9150.025.9754241
TCTGTCG10200.024.7056798
GTACTAC3551.6916601E-1024.3457241
CTGTTGC20600.024.1163089
GTCTCAC20100.023.6492671
CTCGCTC10050.023.6421433
GTGTAAC6150.023.4220391
TAACACT10150.023.4086764
CGCTCTG10550.021.8386825
TCTCGCT11650.021.6312242
GTTAGCC10000.021.6003233
TAATACT6350.021.543134
GGCTAGA11550.021.2015081
CTTGCTC21750.021.1865233
GTGCAAC7150.021.1535421
TTGCTCT24500.020.8651354
GGCTGGA57500.020.792661
Produced by FastQC (version 0.11.5)