FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246516
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11400.043.6199381
TCTGTCG11450.042.75728
TCGCTCT12850.036.4505584
CTGTCGC15600.035.0747689
TCTCGCT14050.034.875992
GTCTTGC26050.033.1981851
CGCTCTG14200.029.9187265
CTCGCTC15550.029.1947083
GGATACG3358.0035534E-1125.8153061
TAATACT9300.025.569664
GTGTAAC8800.025.3874911
TCTGTCA37200.025.1597448
GTCTCAC31850.024.4374071
GTCTTAC12500.023.6382121
TTGCTCT36750.023.529784
GCGTTAC2202.3363522E-522.9306371
GTACTAA6300.022.8786431
GTGTAAA19000.022.3789251
GTGTTAC11700.022.1746851
GTATTGC8150.022.1066651
Produced by FastQC (version 0.11.5)