FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246516
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12650.035.86421
GTCTTGC24950.035.501421
TCTGTCG14850.031.5150368
TCGCTCT14400.029.9998864
GTCTTAC12400.027.8760151
CGCTCTG15500.027.406355
TCTCGCT17200.026.3724022
CTCGCTC17450.025.9945743
CGTTTCG1959.246154E-625.846069
GTCTCAC31500.025.604641
GTGTTAG18150.024.2027281
GTATAGA10700.022.8826481
GTATTAG8900.022.6557881
GTGTTAC12950.022.243411
GTATTAA9400.022.2167871
GTGTAAG13650.022.1578581
GAGTGCA67750.021.7858986
TTGCTCT35850.021.489464
CTGTCGC23300.021.3218089
ACCGTGT10150.021.2807088
Produced by FastQC (version 0.11.5)