FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5690183
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG21400.042.0542538
TCGCTCT24450.033.8644564
GTCTCGC22800.033.818321
CTGTCGC27200.032.2927179
GTCTTGC47300.030.9270171
TCTCGCT26800.028.5019252
CTCGCTC28400.026.6424473
CGCTCTG28500.025.262735
GTGTAAT23900.023.819481
GTGTTAC23000.021.305131
TTGCTCT64550.021.1925134
CTTGCTC62800.020.7693083
GTGTTAG38300.020.6965051
GTATTAG20550.020.689181
GTGTAAG28350.019.5722521
TACACTG32400.019.5552235
GTATAAT19550.019.5358471
GTATTAC13650.019.5331441
TTACACT25400.019.2752654
TATACTG18750.019.1996755
Produced by FastQC (version 0.11.5)