FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6202517
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG21200.040.7532048
GTCTCGC24300.039.4374161
TCGCTCT25250.037.0685274
TCTCGCT28000.032.682012
CTGTCGC29050.031.9712739
GTCTTGC50500.031.390221
CTCGCTC28900.030.4176123
CGCTCTG29000.028.7993955
GTATAAC10650.023.679991
TACACCG5200.023.5379665
TTGCTCT66200.023.0569154
GTCTCAC59700.022.9320221
GTGTTAC22800.022.1220971
GTCCTAC15800.021.8900761
CTTGCTC65350.021.5006753
GTCTCGA48300.021.1667446
TCTGTCA69300.021.0901268
GGGTTTC82200.021.0379071
TCGAACT32950.020.9764589
GTGTTAG38500.020.9614051
Produced by FastQC (version 0.11.5)