FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6202517
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC24350.034.3242381
TCGCTCT27250.031.4397494
TCTGTCG25800.029.3007478
GTCTTGC50900.028.8771461
CGCTCTG27150.028.6384185
GTATTAG18950.026.2350391
GTATTAC13000.026.049321
CTCGCTC31450.025.8678233
TCTCGCT30650.025.3887982
GTGTAAC18050.023.9505751
GTATTAA17200.023.8774681
GTCTAGC23050.021.8810921
GTATAAC12150.021.3485361
GTATTGG20900.021.0293271
TTACACT26250.020.8440884
GTCTTAC21500.020.7775861
CTGTCGC37450.020.76249
TAATACT16700.020.6929974
TTGCTCT67800.020.6001634
GTGTAAG28500.020.477741
Produced by FastQC (version 0.11.5)