FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809586
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG30800.043.7122468
GTCTCGC33050.042.9511151
TCGCTCT36150.036.24834
GTCTTGC69550.033.7753681
TCTCGCT40650.033.6800462
CTGTCGC42800.033.3068589
CTCGCTC40550.031.63073
CGCTCTG40750.028.6227915
GTGTTAC33600.025.0914781
TTGCTCT96550.024.7577024
CTTGCTC92750.023.5401023
GTCTTAC33850.023.2031781
GGGTTTC119250.022.478341
GTATTAG26750.022.3580511
GTTTCAC111600.021.6947543
TTACACT34750.020.9262374
TCTTGCT107500.020.7793942
TTACTCT44700.020.7781434
GTACTAG17450.020.6468721
GTCTAGC33300.020.5569671
Produced by FastQC (version 0.11.5)