FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864510
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC27750.038.6938171
TCTGTCG26550.038.235318
TCGCTCT29550.033.137034
GTCTTGC54600.031.1485371
TCTCGCT32850.030.9315762
CTGTCGC35250.029.4112429
CTCGCTC33250.029.0423263
CGCTCTG35300.024.2719355
GTCTCAC68700.023.4968431
GTGTAAC22550.023.0093441
GTCTTAC23650.022.8532711
GTGTTAG43550.022.1735341
TTGCTCT76650.022.0743454
GTGTTAC27200.020.9303471
GTATTAG25350.020.1836011
GGAGTGC136300.020.073355
GTATTAA20500.020.0373021
CTTGCTC72500.019.9791183
GTGTAAG33600.019.946281
GTATAGA23850.019.9422341
Produced by FastQC (version 0.11.5)