FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9997830
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC42300.038.16431
TCTGTCG36100.037.095098
TCGCTCT42800.036.671424
GTCTTGC82300.034.0642931
TCTCGCT49250.032.047012
CTCGCTC48900.031.9816233
CTGTCGC46450.030.6895229
CGCTCTG50350.028.8846365
GTCTTAC33900.025.7238121
GTCTCAC96450.025.0318071
TTGCTCT108350.024.0544074
GTGTTAC34200.023.3908771
CTTGCTC105300.022.4489023
TCTGTCA109750.022.1073178
GTGTAAA59500.021.8024431
GTGTAGC40900.020.9687841
GTCCTAC24300.020.4640881
TCTTGCT125400.020.4598312
GTGTTAG64700.019.9387721
CTGTCAC123550.019.929399
Produced by FastQC (version 0.11.5)