FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9997830
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC40900.031.8837911
GTCTTGC80700.031.2470631
TCTGTCG44300.030.2273548
TCGCTCT44000.026.8337944
TCTCGCT52950.023.1326792
CTCGCTC49650.023.0551973
GTCTCAC101500.022.998191
CTGTCGC62700.022.504919
CGCTCTG48550.022.094145
TAATACT32400.021.9978924
GTATTAG33950.021.8580861
GTGTTAG59650.021.7408621
GTCGTAC5350.021.5467071
GGAGTGC197850.021.5409935
TTGCTCT111900.021.1668764
GTGTAAG44650.021.1380311
GTATTAA30000.021.1337281
GTGTTAC36450.020.9518831
GGTATAC19800.020.7407461
GAGTGCA217350.020.5358226
Produced by FastQC (version 0.11.5)