FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482933
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4600.028.9115948
GTATATA2550.024.7129761
TGTCGCC5300.023.11205110
AATACGA800.002667762621.87464125
CTGTCGC5550.021.4403679
GTCGCCC5400.021.38781711
TCGCTCT5000.021.0046124
GTCTCGC4700.020.8570521
GTCTTGC8800.020.6877571
GTATTAG3500.020.0057431
GTATAGG2203.4924597E-919.0963921
TCGCCCA6250.018.47907412
CGCTCTG5200.018.177075
GTGTACG1000.00967565117.5050261
CTACGCT1000.00967940917.5038454
CATGCGA1401.866853E-417.49912510
TTGCTCT10500.017.3371414
GTATTAA3650.017.2652321
TATTAAG2651.877197E-917.1741642
TAATAGT2451.3254976E-817.1466244
Produced by FastQC (version 0.11.5)