FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482933
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3950.031.0122538
GTATAGA2250.028.0062451
TTGTCGA500.00721149127.99963661
GTCTCGC3850.026.3695161
TAGCGCT1101.0173535E-625.4542144
ATACCGT700.001226815924.9996766
TGTCGCC4850.024.53576310
GTCGCCC5000.023.79969211
GTATAGG2103.6379788E-1223.3385371
CTGTCGC5250.023.333039
GTCTTGC8750.022.8050861
GTATAAT1807.2559487E-921.3936611
CGCCGTA1356.0272796E-620.7404768
TCGCTCT5100.020.5879694
GTGTTAG6100.020.0864471
CGTGTTA2101.9663275E-919.99973910
TCGCCCA5900.019.57601712
GTCTAGC4600.019.0259821
TAATACT2456.6211214E-1018.5711864
ACTGTCG950.00721210818.4208138
Produced by FastQC (version 0.11.5)