FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529554
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGT604.9805717E-429.1711481
TCTGTCG4150.027.8310558
CTGTCGC4800.025.5205849
TGTCGCC4400.025.45429610
GTCTCGC5150.024.469781
TCGCTCT4900.023.575824
GTATAGA2450.022.8606551
GTCTTGC8800.021.8783631
TCTCGCT5350.020.9377982
GTCTATA1709.310679E-820.5913981
GTATTAG2900.020.5203931
CTCGCTC5500.020.3667663
GTATAAT2453.092282E-1120.0030731
TATACTG2653.6379788E-1219.8150145
CGCTCTG5500.019.730955
TATATAC3500.019.0029183
GTCGCCC4850.018.76208911
GGTATAC1702.1175983E-618.5322591
GTGTTAG6550.018.170731
GTGCGAG1752.7933092E-618.0027661
Produced by FastQC (version 0.11.5)