FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529554
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT200.00695226952.50039321
CGGTACT500.00720968928.0011254
CCGCTAG500.007210839528.000219
GCGTAGT657.9556403E-426.9259171
GTCTTGC9700.025.2603991
TGTCGCC5100.024.01978710
GTACTAC1502.4770998E-823.3357941
GTATAGG1953.092282E-1123.3357941
TCTGTCG4500.023.333518
CTGTCGC5250.023.3335089
GTATTAA2451.8189894E-1221.4308321
GTATAAA3700.020.8130071
GTCGCCC5400.020.09274311
GTATTAG3400.019.5608881
CGCCGTA900.005277419519.4445967
GTATAGA2353.783498E-1019.3637471
TAATACT2002.4427209E-819.2507744
TCGCCCA5650.019.20368412
GTGTTAT2951.8189894E-1218.9850541
CGTATCA1150.001008174518.26100770
Produced by FastQC (version 0.11.5)