FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325052
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC1601.8189894E-1228.440781
GTCTCGC5150.026.5079121
TCTGTCG4950.025.4536428
GTCGCCC5150.022.42638611
GTAATCG1253.0935753E-622.39920270
CGTGTTA1102.9246987E-522.27193510
TGTCGCC5250.021.99921810
TCGCTCT5600.021.8775254
CTGTCGC5800.021.7233649
GTATTAG2451.8189894E-1221.4310441
TCTCGCT5850.020.9425872
GAATAAC2051.4533725E-920.4901681
GTGTAAA3650.020.139311
CTATACT1751.264325E-720.0023084
TATAATA1751.264325E-720.0023082
TCGCCCA6350.019.84331112
GTGTAGT3750.019.6022621
GTGTTAG5400.019.4466881
GTATAGC1801.7012644E-719.4466861
TTACGCC900.005273764519.4466864
Produced by FastQC (version 0.11.5)