FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325052
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4350.028.964888
CTGTCGC4750.027.9993829
TGTCGCC4750.027.26255610
GTCGCCC4900.025.71371711
GTCTCGC4650.024.8437861
TCGCCCA5500.022.27223412
GTCTTGC9150.022.1903251
AAACCGT800.002667702321.8745166
TCTCGCT5050.020.7939742
TCGCTCT4500.020.2217754
TTATACT2302.8376235E-1019.782174
GTCTTAC2705.456968E-1219.448421
GTATAAT1801.6996819E-719.4484181
GTATTAG2002.4372639E-819.2539351
TACACGT1301.0579713E-418.8457375
GTACTAG1501.494121E-518.670481
GTCTTAT3001.8189894E-1218.670481
CGCCGTA950.0072120918.42064568
GTATAGC2104.263711E-818.337081
CTCGCTC5750.018.2611563
Produced by FastQC (version 0.11.5)