FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968637
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG2550.034.312138
GTATAAG1900.031.3191851
CTGTCGC3250.028.0001459
CGCGGCA500.007211453727.998712
GTCTTGC5400.027.2251761
GTATAGG1451.7267666E-824.1405491
GTCTCGC2950.023.7313861
GTACTAA1202.1663145E-623.3358611
GTATTAG1854.0381565E-1022.7051641
GTATATG1703.752575E-922.6495151
TGTCGCC3400.022.64717510
TCGCTCT2950.022.5459824
GGTATAC1253.0882857E-622.402431
TCTCGCT3300.022.2751432
GCGAAAC800.002668365421.87285626
TAATACT2259.094947E-1221.7812634
GAGCCGT1154.113568E-521.3077586
CTCGCTC3300.021.214423
TAGTACT1003.928766E-421.0033634
GTCGCCA1356.0254697E-620.73977711
Produced by FastQC (version 0.11.5)