FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199581
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3550.026.6191968
GTATTAT2000.026.250581
GTCTCGC3600.026.2505761
CTGTCGC4250.024.7064259
TCGCCCA4250.023.88287712
GTCGCCC4350.023.33384711
TGTCGCC4500.022.55605110
TCGCTCT3800.022.105754
GCCGACC2108.367351E-1121.66624370
TATACTA1003.9331836E-421.0004632
CGCTCTG4350.020.925
TCTCGCT4200.020.8337922
GACTCGT850.00379129120.5878316
GTTCTAA2750.020.3640841
GGGTATA2101.9645086E-920.000441
TACACGG900.00527632519.4448725
CTCGCTC4350.019.310773
GTATAGA2203.5015546E-919.091331
GTCTTGC6800.019.0445371
GTCTCAC9100.018.846571
Produced by FastQC (version 0.11.5)