FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396389
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3600.032.081768
GTCTCGC4600.028.921981
TGTCGCC4100.026.46211410
CTGTCGC4600.025.8682969
TCGCTCT4350.024.9502374
GTCGCCC4300.024.41740611
TAGTACT1902.1827873E-1123.954774
TCTCGCT4750.023.5862372
TAGATCG750.001832987323.33385770
ATCTCGT3650.022.05529470
TTACTCT5100.021.9675734
CGCTCTG4700.021.602425
CTCGCTC5100.021.2810863
GTATTAG2650.021.1386071
GTATTAT2551.8189894E-1220.5945991
GTATTAC1558.726074E-720.3288631
GACTCGC1558.750103E-720.32303870
GTATAGG1951.8206265E-819.7496911
TCGCCCA4850.019.4835812
CGTGTTA1702.1214382E-618.52850210
Produced by FastQC (version 0.11.5)