FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396389
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3700.025.5394958
GTATTAG3100.024.8412511
GTACTAG1301.6065678E-724.233251
GTCTTGC8400.024.1691381
CTGTCGC4400.023.862669
GTATAGA2700.023.335721
TGTCGCC4250.023.05787810
GTGTAAA5450.022.4793641
TCGCCCA3950.022.15099112
CGTGTTA1905.638867E-1022.10435910
GTCGCCC3700.021.75586711
GTCTCGC2750.021.6385781
GTGTTAC3600.020.4187551
ACCGTGT3200.019.6866958
GTCTAAT2152.6302587E-919.5368821
TTGCTCT11550.019.090814
GTTTCAC11500.018.8708213
GTATAAT2851.2732926E-1118.4229371
GTGTAAC3250.018.3095661
GTGTTAG5650.017.966441
Produced by FastQC (version 0.11.5)