FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259567
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3150.029.9993348
TCGCTCT4100.029.023754
GTCTCGC5000.028.0038261
GTATAGG1900.025.7930011
CGACGAA1752.0190782E-1023.99946870
CTCGCTC4900.023.5709083
GTGTTAC2600.022.8877451
TCTCGCT5200.022.8859252
CTGTCGC4150.021.9272259
TAGCACG1205.7192225E-520.4162164
GTCTTAC2750.020.3664191
CCCGATC1055.467774E-419.9995578
GTACTAG1257.8189056E-519.6026781
TGTCGCC4450.018.87598610
ACGTGTA7450.018.7915370
TATACTA1501.49694315E-518.6662545
GTGTTAG6050.018.5149271
CGATTTC950.007210244418.42137714
GTCTCAC9550.018.3271121
TGCGCAA1150.001008240618.26046610
Produced by FastQC (version 0.11.5)