FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122812
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC1700.032.940770
TCTGTCG4600.022.8262948
GTCTCGC5250.022.6690081
GTATAAT4050.020.7428841
GTGTAAG6250.020.1620831
CGCCGTA1257.831219E-519.59971668
TACCCCG1801.7043749E-719.4446225
GTGTAGG8300.018.9778631
GTATTAG5100.018.5313261
GCCGACC3400.018.52914470
GTATTAC3900.017.9505711
TCTCGCT6150.017.6420212
GTATAAA5600.017.5018081
TACACCG1000.00969248917.5001585
GTCTTGC11850.017.4279611
CTGTCGC5450.017.3396079
GTATAAC2259.399264E-817.1128791
GTTAGCC5750.017.0432323
GTGTACG1653.39487E-516.971451
TCGCTCT5400.016.8516084
Produced by FastQC (version 0.11.5)