FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063029
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGCC3200.036.0934610
CTGTCGC3300.034.9997189
TCTGTCG3300.031.8179268
GTCTCGC3000.031.5041921
TGCGACA450.00430848831.1108643
TCGACCG500.00721041327.9997739
GTATAGG1653.6379788E-1227.5794281
TCGCCCA3750.027.06644812
TCGCTCT2850.027.0173264
GTCGCCC3500.026.9997811
GCGTAGG700.001225543425.0033251
CGCTCTG3800.023.9471765
CTGCGCG750.001833026223.3331479
TAACACT3250.022.61524
GGTATAC1253.0880965E-622.402981
TCTCGCT3450.022.320762
GTATTAA2401.8189894E-1221.877911
CTCGCTC3600.021.3887163
TATACTG1859.966243E-920.8106425
GTGTAAC2551.8189894E-1220.5909771
Produced by FastQC (version 0.11.5)