FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071659
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG2250.035.7787328
GTCTCGC2750.025.45761
CTGTCGC3750.023.3339589
TGTCGCC3600.023.33395810
TCGCTCT2850.023.3339564
TACACTG4550.022.3082875
GTGTAAC1755.2441465E-922.0026381
CTTGTCG800.002666617321.8755849
GTCTTGC7350.020.9548951
GTCGCCC3550.020.70477911
ACCGTTC850.00379001420.5887858
AGGTCCG850.00379001420.5887855
TTACACT3300.020.1520524
ATACACT2101.9626896E-920.0005344
GTGTATA3400.019.561171
GTACCCG900.00527297719.446781
GTATAGA1801.7004277E-719.446781
TCTCGCT3250.019.3860382
CTAGTAC1451.11634745E-519.311763
TTGCTCT8900.019.2701764
Produced by FastQC (version 0.11.5)