FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426308
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6300.024.4447698
GTCTCGC6200.024.2785191
CTGTCGC7500.023.3336439
GTACTAT3350.021.9442081
GTCTTGC13850.021.7367251
GTCGCCC7350.021.42885411
TGTCGCC8250.020.78815310
TGTACGA850.003791670520.5885072
GTGTTAC6000.020.4203031
GTATAGG4000.020.1285861
GTATTAG4750.019.8982811
TCTCGCT7050.019.8584182
GTGTATA8700.019.3137861
GTACTAA3650.019.1814981
CGTGTTA2405.0386006E-1018.95858610
TCGCTCT6900.018.7679794
TCGCCCA7950.018.4908112
GTACGAT1351.4113344E-418.1513821
GTATAGA4450.018.0931111
GGGTAAT4550.017.695461
Produced by FastQC (version 0.11.5)