FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290313
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA2500.029.39992570
TCTGTCG6350.028.1101678
GTATTAA2750.022.9120351
CTGTCGC7650.022.4182459
GTCTTGC14150.022.2643471
TAATACT4250.021.4117134
GTATAGA3900.020.6436791
TGTCGCC8500.020.58818410
GTCGCCC7700.019.54540611
GCCGACC3450.019.27531470
GTCTCGC6150.018.7829021
CGTATTC950.007212840518.42100514
GTCTAAC3203.6379788E-1217.5022491
ATAGTAC2802.0736479E-1017.5003383
ACGTCGG1000.00969336117.49995830
GTACTAT3650.017.2624911
GTATAAT3650.017.2624911
GTATTAC3450.017.2485921
GTTTCAC20100.017.2391383
GTGTAAA8400.017.085531
Produced by FastQC (version 0.11.5)