FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761452
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7500.023.807551
TCTGTCG7850.023.6299368
TCGCTCT8550.021.2925324
TATACTG4650.021.0811925
TGTCGCC8800.020.28354610
GTATAGT4200.020.0063461
TAGACCG1055.458881E-420.005625
CTGTCGC10000.019.9494639
GTATAAC3200.019.6937451
GTCTTGC15650.019.6867541
ATCTCGT8250.019.09039570
CTCGCTC9000.019.0609113
GTCGCCC8350.018.44261611
GTACTAG3250.018.3135011
GTGTATA8700.018.1091921
GTATTAA4900.017.8628081
CGTGTTA3153.6379788E-1217.777310
TCTCGCT9100.017.6972792
TAAATCG1000.00967804417.504925
TCGCCCA9200.017.49952912
Produced by FastQC (version 0.11.5)