FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350450
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4700.026.0629678
CCGATCG851.3098835E-424.7050639
TGTACGA750.001830096123.3410022
GTCTCGC6100.022.9603181
GTTCCGA1003.9243416E-421.008691
GGTATAC3550.020.7127931
GTATAAG4400.020.6903761
TAATACG850.003785181620.5945644
GTATAGG4150.020.2493381
GTATTAC3150.020.0082761
GTACTAC2801.0913936E-1118.757761
GTCTTGC14550.018.531
CTGTCGC6800.018.5287979
GTGTACG1351.4087667E-418.1556571
CGCTCTG6950.018.1350695
TAATACT4250.018.1232174
TACCCCG1752.7905062E-618.0055335
TGTCGCC6850.017.8826210
GTCTTAC5800.017.5072421
GCGTAGT1200.001337918917.5072421
Produced by FastQC (version 0.11.5)