FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350450
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG150.00222522969.9997255
CGCGAAT500.00721201827.99989114
GTACCGA700.001225291825.0057531
TCTGTCG5650.024.1591978
GTCTCGC7250.024.1434861
GTATATA5450.022.4822351
GTATTAT3900.021.5434171
ATACGCC1154.121314E-521.3047183
GTCTTGC14050.020.6809141
TCTCGCT7600.020.2635082
TCGCTCT7850.020.0636144
CTGTCGC6700.019.8506689
GTATTAC3400.018.5336741
GTGTAAG8300.018.1367031
TGTCGCC7150.018.11181610
CTCGCTC8700.018.1037623
GTCGCCC6800.018.01463311
ATAGTAC2751.6007107E-1017.818493
GTGTAGG7300.017.7438091
CGCTCTG8350.017.604725
Produced by FastQC (version 0.11.5)