FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604678
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7100.029.082838
GTCGCCC6800.027.7930511
TGTCGCC8100.027.22117810
CTGTCGC8450.025.6794879
GTATTAA5350.025.52481
TCGCCCA8050.024.78165612
GTATCGA750.001829150423.3431931
GTACTAT3200.020.790031
ATCGCGT850.00379287820.58744446
GTCTCGC7900.020.3883571
GTCTTGC15850.019.6613031
ATACCGT900.00526925619.4504186
GCGAATT900.00527979419.44369913
TCGCTCT7650.018.7642944
GTATTAT4200.018.341081
CTATGCG1351.4137279E-418.1474519
GTGTTAC6800.018.0223181
TATCTCG7900.017.72117870
GTATTAG5200.017.5073951
GTTCTAC5050.017.3340531
Produced by FastQC (version 0.11.5)