FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668903
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.038.1489148
GTATTAA7300.031.7984121
CTGTCGC14250.030.3069849
TCGCTCT12200.030.0946924
GTATACG2403.8578946E-828.080051
GTCTCGC10700.027.2927571
GTCTTGC22050.027.1673951
TCTCGCT13150.025.1978782
GTACTAT6150.024.3512611
GTCTTAC9650.024.0547561
CGCTCTG13300.023.8126325
GTATAAG8200.023.742481
GTATTAC6750.023.296041
GTGTAAA16250.022.5792371
TCGATCT9900.022.5389049
CTCGCTC14100.022.4653453
GTGCAAC9250.021.8569031
GTATAGA9450.021.3943231
GTGTTAC9950.021.0717951
TAACACT13050.020.9629614
Produced by FastQC (version 0.11.5)