FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625582
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9600.035.239838
GTCTCGC11800.031.7280481
TCGCTCT12700.030.0454444
GTCGTAC1050.0065866228.5250241
TACACCG1804.975109E-627.9919245
GTGTAAA14050.027.7129251
CTCGCTC13700.026.8013133
GTCTTGC23650.025.9619941
TCTCGCT14800.025.7969822
CTGTCGC13900.024.3383039
GTATTAC6500.024.1914161
CGCTCTG14500.023.33125
TCGATCT10500.023.3075629
GTCCTAT6800.023.1241471
CTTACCG1600.00226917922.4935119
GTATTAG10000.022.4634591
CTCGATC9750.022.1474578
GTCTCAC28100.022.1170461
GTACGAA2052.9496822E-421.9155671
TCTGTCA33650.021.818378
Produced by FastQC (version 0.11.5)