FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728038
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.048.206968
GTCTCGC11450.045.727731
TCGCTCT12500.045.4998554
CTCGCTC13550.038.7861373
CTGTCGC14550.037.597939
TCTCGCT14900.035.7708742
GTCTTGC23350.033.634851
CGCTCTG15100.031.9382575
GTCTTAC10450.027.199091
GTATAAA10050.025.3046261
GTCTAGC12000.023.6858731
TTGCTCT32300.023.403444
GTTAGCC14200.023.3218213
GTGTTAG18050.023.205871
GTACTAC4850.023.1332471
GTATAGT7500.021.94061
GGAGTGC63600.021.8430635
TCTAACG1650.002703179121.8257982
GAGTGCA69100.021.7711626
GTGTATA14200.021.596461
Produced by FastQC (version 0.11.5)