FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728038
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.035.530258
GTCTCGC11000.032.7433131
TCGCTCT13550.030.8103684
GTCTTGC23500.027.7795621
GTCGTAA1656.665977E-527.286091
CTGTCGC17000.027.0981249
GTATTAC5900.026.7079961
CGCTCTG15550.026.3847265
GTATTAA8200.025.6223051
CTCGCTC14300.025.167623
GTATAAG6800.024.2765961
GTCTCGA23300.024.0961046
GTGTTAC10000.024.0117631
TAATACT8150.023.8459344
GGAGTGC60200.022.7177475
CTAGCGC2252.8056234E-522.3935893
GAGTGCA66300.021.9303726
ACCGTTA1650.002713166621.8119378
TCGATCT9250.021.7883559
GTGTTAG18700.021.6683671
Produced by FastQC (version 0.11.5)