FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2532501
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC4750.036.331581
GTCTCGC11000.032.7415051
GTATTAA7650.032.367011
TCTGTCG10650.031.0920988
ATACCGA1902.212746E-730.309466
GTATTAC6000.030.0130421
GTACTAG4050.025.9371991
TCGCTCT12500.025.3352054
TCTCGCT15050.024.8738462
GTGTTAG17000.024.7166251
GTCTTGC21850.024.3813061
GTTAGCC13950.023.7336963
CTCGCTC14050.023.5647723
GTATAAC5150.023.3111021
GTGTAAC8850.022.8913061
GTACTAC5000.022.5097831
GTGTTAC10550.022.0474971
GTACTTA4859.094947E-1221.6588971
CTGTCGC13900.021.2329759
GAGTGCA61150.021.0716426
Produced by FastQC (version 0.11.5)