FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366797
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.035.989458
GTCTCGC10600.033.883411
TCGCTCT11750.033.0838554
CGCTCTG13050.029.784375
CTGTCGC12100.028.5536129
GTATAAT7600.028.5519751
TCTCGCT13300.026.536482
CTCGCTC13050.026.4783543
GTATAAG8850.026.2101921
GTCTTGC21000.025.6545851
GTCTAAC5950.025.1515521
TTGTCGC2701.770004E-723.9929649
GTGTTAA10400.023.7428251
GTGTAAT10400.023.7428251
GTATTAA7250.023.7378621
GTACTAC3802.4556357E-1023.6292211
GTTCTAT8500.022.0076071
GTATTAG9000.021.6163621
TATACTG8300.020.8131755
GGGTAAC8650.020.7609331
Produced by FastQC (version 0.11.5)