FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804355
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.040.587668
TCGCTCT12700.035.1409384
CTGTCGC14500.032.763179
CGCTCTG14150.028.995355
CTCGCTC14050.028.1781063
GTCTCGC11400.026.9158421
GTCCTAC7800.026.8653451
GTATTAT10450.026.4980811
GTCTTGC25150.026.4838431
GTATAAG7800.025.9058691
TCTCGCT14850.025.2274952
GTCTAAC6400.024.5566041
GTATAAA11600.022.5807861
GTATTAC6300.022.5705451
TCTAACG1600.00225740622.5137212
GTATAAT8200.021.9041481
GTACTCG2403.4180288E-521.8280931
TAATACT8100.021.3280744
GTGTAAA16500.021.3178251
GTACAAA14550.021.0887031
Produced by FastQC (version 0.11.5)