FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804355
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10550.035.590321
TCTGTCG11100.032.4233138
TCGCTCT13700.030.9969254
GTCTTGC23250.030.0382331
TCTCGCT15500.027.8681012
GTATCCG1356.583826E-427.8131771
CTGTCGC15050.025.8266249
CTCGCTC15000.024.4718113
GTATAAA11600.023.3055251
GTCTTAC10650.023.2690531
GTACTAC4900.022.9884411
GTACTAA5600.022.7968731
GTATTAA9300.022.609421
TGGACCG3203.6614438E-822.4928745
GTCTCAC29450.022.4394261
CGCTCTG15450.022.3618475
GTATTGG9500.022.1334321
GTATTAG10650.021.8588091
GTGTAAA19100.021.6243821
GTATTAC7300.021.6028371
Produced by FastQC (version 0.11.5)