FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330238
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9050.039.7688758
GTGTAAC7100.031.4262751
TCGCTCT10800.031.32674
GTCTCGC9100.029.4232831
CTGTCGC13600.026.4638489
CTCGCTC11850.026.121053
CGCTCTG11300.025.4802365
GTCTTGC20500.024.6709041
CGGTAAC1550.00155930523.9921041
GTCTAGC9650.023.8926541
GTCCCGA3752.237357E-1023.8001671
TCTCGCT12000.023.409112
GTATAGA7050.023.2093811
GTACAAA10950.021.7353131
GTGTAGC9700.021.4692231
GTGTAAA13600.021.3282761
GTCTCAC24150.020.9421751
TTGCTCT27550.020.9030134
GTGTTGC14700.020.7441921
GTACCAA5800.020.5173841
Produced by FastQC (version 0.11.5)