FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330238
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10150.043.3684581
TCGCTCT12850.038.089594
TCTGTCG10250.037.9250648
CGCTCTG13350.033.4287575
TCTCGCT14750.031.7259772
GTCTTGC21000.031.6197741
CTCGCTC14100.030.6289843
CTGTCGC15300.027.759849
TACTGCG2654.9240043E-927.1650497
GTGCTAC7350.023.3468631
GTGTTAC8400.023.0930941
GTCTCAC21350.023.063971
GTATAAA8650.022.4256631
GTCTTAC8350.022.337861
CCGTGTT7500.022.076139
ACCGTGT6200.022.060658
GTATACG2403.500101E-521.76081
GTATTAG7950.021.5848371
GTATTAA6600.021.4781911
GTATAGA7750.021.1791741
Produced by FastQC (version 0.11.5)