FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488755
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC300.001990300471.995533
CTTAGTA350.00366688161.710462
TGTAAAC1157.495688E-637.5628852
TATAAGA1604.883259E-835.9977652
GTATAAG1957.4487616E-933.247691
GTACTAC1402.8705757E-530.8728561
TGAATCA2202.4248948E-829.4527195
TAATTGT1000.006286205328.7982123
TAAGAGA2301.1729662E-625.0419244
GTGTAAA1550.001877637723.2376351
TGTATTG1952.7420878E-422.152475
ATAAGAG2704.7246504E-621.3320083
TATACCG2704.7246504E-621.3320085
TTACTCG1700.00322133221.1751564
TTACGGT3406.929804E-821.1751564
GTATTAC2053.8298772E-421.0839021
TTATAGG2404.606007E-520.9986972
GTTATAG1750.003798043820.5819031
CTGTAGT1750.003810630620.5701522
ATGTGTA1750.003810630620.5701529
Produced by FastQC (version 0.11.5)