FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523526
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9900.039.2614148
GTCTTGC19900.035.6892551
GTCTCGC9200.030.0663571
TATACCG1000.006293969728.7917025
GTCGTAA1305.4198824E-428.753691
CTGTCGC14600.027.6084849
GGTCTAC5150.027.581211
CTCGCTC11500.027.5426183
GTACTAG4700.027.0407051
GTGTAAC7050.026.5104941
TCTCGCT11950.025.9189722
TCGCTCT11950.025.9030514
GTACATA7700.025.24351
GTCTCAC25950.024.7758221
GTCTTAC8800.024.6366861
TTGCTCT31650.024.1091844
CTTGCTC31350.023.8806513
TCTGTCA28800.023.7431568
CTCGATC9400.023.737848
TCGATCT9750.023.623969
Produced by FastQC (version 0.11.5)