FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523526
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.034.4529048
GTCTCGC10250.033.6413541
TCGCTCT11300.031.2103234
CTGTCGC13600.029.6396319
CGCTCTG11500.028.7905035
TCTCGCT13550.027.6251682
GTCTTGC20400.026.4570751
CTCGCTC13250.026.0744173
TATACTG7050.025.5234955
GTACTAG3401.7043931E-924.252321
GTGTAAC6650.022.5448761
GTACAAA10450.022.2374461
GTCTAGC9500.022.0939771
TACACCG2303.3254466E-521.9058155
GAGTGCA57300.021.8583956
GTATTAG7950.021.6870371
GGAGTGC53900.021.6329385
GTCTAGT10050.021.6307431
GTCTCAC26400.021.5798851
GTATAGA8050.021.4176311
Produced by FastQC (version 0.11.5)