FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653646
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.040.8530738
GTCTCGC12700.040.719591
TCGCTCT12850.040.3383754
TCTCGCT14950.038.547092
CTCGCTC14050.036.89313
TACCGAC900.003759703431.9907676
CGCTCTG15100.031.9378035
CTGTCGC14300.031.2077759
GTCTTGC22550.029.5802251
GTACTAG5200.027.3847161
GTATAGC5100.024.9825481
CGCGTGA2101.2032942E-524.9825481
GTGTAAC8800.024.6986561
GTATTAA7650.024.4926951
GCGTAAA1600.001794008923.421141
GTGCGAA2002.4803466E-422.4842931
GTCTAGC8700.022.3981461
GTATTAC6050.022.2984731
CGGTAAC1700.002551830122.0434271
GTACTCG2453.9626277E-521.4136141
Produced by FastQC (version 0.11.5)