FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653646
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10700.037.9270361
TCTGTCG11750.036.7555858
TCGCTCT11550.035.5211034
GTCTTGC23400.034.0430871
GTACCGA900.003043657933.4007641
CTGTCGC15950.028.8821339
TCTCGCT14250.028.2920862
CTCGCTC13800.027.643333
GTATAAG7200.026.0943471
GTGTAAC6700.024.6766851
TTGCTCT33700.024.5618744
CTTGCTC31650.023.8786183
CGCTCTG13300.023.811935
GTCTTAC11200.023.4849151
GTCCTAC7950.022.6873111
CTGTTGC25400.022.1039989
GTATTGG7850.022.0189761
GTCTCAC28350.022.002091
GTGTTAG16550.021.796271
GCGCAAA2103.386948E-421.4719181
Produced by FastQC (version 0.11.5)