FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040234
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8150.043.2879758
GTCTCGC9300.037.934231
GTCTTGC17800.037.2122541
TCGCTCT10550.036.1703074
CGCTCTG9750.033.2304885
CTCGCTC11650.030.281523
TCTCGCT11800.029.8965842
GTATTAA4950.029.0899491
CTGTCGC12500.028.223769
TTGCTCT24900.027.4696454
CTTGCTC22750.027.2160173
GTCTCAC22950.025.0972121
GTATAAG6350.024.9440571
TAACACT10150.024.8273744
GTATAAC4400.024.5446431
TCTGTCA24600.024.2924778
TCTTGCT27550.023.258192
ATCCGTT1550.001883537323.2256097
GTGTTAA7500.023.0392381
GTGTACG2852.974648E-722.7360921
Produced by FastQC (version 0.11.5)