FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248073
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.034.24818
GTCGTTA900.003753341732.001621
GTCCGGT1652.5195968E-630.5471
GTCTTGC19450.029.9860951
TCGCTCT9200.029.7406374
GTCTCGC9350.028.4934231
GCGTAAA1555.7671336E-527.872381
GCCGTAC1400.00104073825.715591
CTGTCGC13000.025.4765159
GTATAAA7650.024.4718271
ACGGTTC1801.5943858E-423.9996158
TTGCTCT23650.023.1385924
GGAGTGC45550.022.9205195
GTGTTAT7650.022.5893781
CTCGCTC10650.022.3109913
GTATTAA5850.022.154971
GTCTTAA7250.021.8493841
GAGTGCA50100.021.8449346
CGCTCTG9700.021.5263845
TCTCGCT10850.021.23612
Produced by FastQC (version 0.11.5)