FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064716
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10000.045.3664471
TCGCTCT10350.041.0493134
TCTGTCG9250.039.696218
CTCGCTC11300.036.9610063
TCTCGCT12000.036.60522
CGCTCTG12250.033.5043685
GTATACG1551.5457063E-632.5207521
GTCTTGC16700.030.6151281
GGTATAC3800.030.3200991
CTGTCGC13150.029.5657319
GTATTAT5850.027.080771
GTATTAC5100.026.8273411
GTGTAAA10750.025.454781
GTGTTAG13750.024.0906961
GTATTGG7050.023.4926991
GTGTTAC6800.023.2974281
GTATTAG6800.023.2974281
GTCCAGA14350.023.083421
GTCTCAC22650.022.890671
TTGCTCT24800.022.648384
Produced by FastQC (version 0.11.5)