FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064716
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.039.4719738
GTCTCGC8800.038.453511
TCGCTCT11200.036.6427574
GTCTTGC15750.032.91341
CGCTCTG12150.030.8147325
GTCTCAC21650.028.9322451
TCGGTAC1000.00628642428.7985293
TCTCGCT12200.027.7362882
CTCGCTC12050.027.4840723
GTATTAG7850.026.5980111
CTGTCGC16050.025.5700249
GTACCGT1450.001277586424.8269181
GTCTTAC9000.024.7993341
GTCTAGT8450.024.7093961
GTATTCG1751.3170336E-424.6850511
TTACTCT12200.024.1966574
GCGTACA1550.001883746623.2251821
ACCGTGT5700.022.736788
GTGTAAG12050.022.7047841
GTAACGC1600.002265889222.498853
Produced by FastQC (version 0.11.5)