FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095869
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8550.035.369031
TCTGTCG8450.033.232938
GTATTAC4850.031.1757931
GTGTTAC6700.031.1647151
TCGCTCT9550.030.9130044
GTATATA8150.026.5035231
GTCTTGC17300.026.2201041
GTACAGA9950.026.05071
CTCGCTC10150.025.5361673
TCTCGCT11600.024.826832
CGCTCTG11050.024.1101635
GAGTGCA50000.023.9055526
GGCTAGA12650.023.905551
GGAGTGC46600.023.4865045
GGCTGGA61900.023.031091
GTCTAGC7850.022.9303321
CTGGAGT55500.022.8317383
GTCTCAC22800.022.7372341
GTATAAA9200.022.6960431
GTATTAG6700.022.5675531
Produced by FastQC (version 0.11.5)